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Polyclonal Antibody Production


POLYCLONAL ANTIBODY PRODUCTION IN RABBIT POLYCLONAL ANTIBODY PRODUCTION IN RABBIT
   
POLYCLONAL ANTIBODY PRODUCTION IN MOUSE POLYCLONAL ANTIBODY PRODUCTION IN MOUSE
   
ADDITIONAL SERVICES ADDITIONAL SERVICES

Services request form for polyclonal antisera production




PRODUCCIÓN DE ANTICUERPOS POLICLONALES EN CONEJO POLYCLONAL ANTIBODY PRODUCTION IN RABBIT

All procedures and protocols used and the care of experimentation animals are performed according to the specifications established in Real Decreto 21/10/2005, on "Protection of animals used for experimentation and other scientific purposes”.


The antigen will be provided by the customer and the amount needed will depend on the nature of the antigen. We recommend the following amounts:


  • Recombinant proteins: about 2 mg
  • Synthetic peptides: about 3 mg

Standard Protocol for the Generation of Rabbit Polyclonal Sera


  • Animals used: 2 female New Zealand White rabbits (Orhyctolagus cuniculus)
  • Animal weights between 1.8 and 2.0 kg
  • Proper animal acclimatization: the rabbits reach animal facilities one week before the initiation of the immunization protocol

The antiserum induction protocol lasts approximately 12 weeks and includes these highlights:


Day Stage / Activity Protocol Sample Obtained
-1 Obtaining pre-immune serum 7-10 ml of serum
0 Priming (Complete Freund’s Adjuvant) ---
3rd week 1st Booster (Incomplete Freund’s Adjuvant) ---
6th week 2nd Booster (Incomplete Freund’s Adjuvant) ---
9th week 3rd Booster (Incomplete Freund’s Adjuvant) ---
10th week Obtaining serum for titration* 7-10 ml of serum
11th week Final bleeding (if 4th dose is not required) 20-50 ml of serum

* (Titer to be determined by the customer who will decide whether a 4th dose is necessary)


The customer will receive 7-10 ml of pre-immune serum and the serum obtained in the final bleeding (20-50 ml).


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PRODUCCIÓN DE ANTICUERPOS POLICLONALES EN RATÓN POLYCLONAL ANTIBODY PRODUCTION IN MOUSE

Services request form for polyclonal antisera production


All procedures and protocols used and the care of experimentation animals are performed according to the specifications established in Real Decreto 21/10/2005, on "Protection of animals used for experimentation and other scientific purposes”.


The antigen will be provided by the customer and the amount needed will depend on the nature of the antigen. The following antigen amounts are recommended:


  • Recombinant proteins: about 0.5 mg
  • Synthetic peptides: about 1.5 mg

Standard Protocol for the Generation of Mouse Polyclonal Sera


  • Animals used: 4 female BALB/c mouse

The antiserum induction protocol lasts a period of approximately 5 weeks and includes these highlights:


Day Stage / Activity Protocol Sample Obtained
-1 Obtaining pre-immune serum opcional
0 Priming (Complete Freund’s Adjuvant) ---
15 1st Booster (Incomplete Freund’s Adjuvant) ---
30 2nd Booster (Incomplete Freund’s Adjuvant) ---
35 Obtaining serum for titration* < 0.1 ml of serum
37 Final bleeding (if 3rd dose is not required) 1-2 ml of serum

The customer will receive about 250 μl of pre-immune serum and the serum obtained in the final bleeding (1-2 ml).


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SERVICIOS ADICIONALES ANTICUERPOS ADDITIONAL SERVICES

  • Protein A affinity purification
  • Biotin or Peroxidase coupling
  • One-year storage in liquid N2
  • Functional validation in ELISA, Western-Blot, IHC, IF and/or Flow Cytometry techniques

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